Clot waveform analysis in patients with haemophilia A

T. Siegemund; U. Scholz; R. Schobess; A. Siegemund

doi:10.5482/HAMO-14-02-0016

Hämostaseologie, Table of Contents

Hamostaseologie 2014; 34(S 01): S48-S62
DOI: 10.5482/HAMO-14-02-0016

Original article

Schattauer GmbH

Clot waveform analysis in patients with haemophilia A

Clot-Waveform-Analyse bei Patienten mit Hämophilie A

T. Siegemund

¹The Scripps Research Institute, Department of Molecular and Experimental Medicine, Roon Research Center for Arteriosclerosis and Thrombosis, La Jolla, California, USA

,

U. Scholz

²MVZ Lab Dr. Reising-Ackermann and Colleagues, Center of Coagulation Disorders, Leipzig, Germany

,

R. Schobess

²MVZ Lab Dr. Reising-Ackermann and Colleagues, Center of Coagulation Disorders, Leipzig, Germany

,

A. Siegemund

²MVZ Lab Dr. Reising-Ackermann and Colleagues, Center of Coagulation Disorders, Leipzig, Germany

› Author Affiliations

Abstract

Summary

Clot waveform analysis extends the interpretation of aPTT measurement curves. The curve is mathematically processed to obtain information about fibrin formation kinetics including semiquantitative determination of thrombin, prothrombinase and tenase activity. Patients, method: In this study the feasibility of clot waveform analysis for monitoring of haemophilia A was investigated using blood samples from healthy controls as well as haemophilia A patients under various clinical conditions. Results: Thrombin, prothrombinase and tenase activity show a high correlation to factor VIII levels. Tenase activity was found to exhibit a linear relationship to factor VIII levels over a very large concentration range and was able to discriminate patients with severe, moderate and mild haemophilia. Conclusion: Clot waveform analysis is an easy, fast and cheap method to access disturbances in clot formation and can be done without any additional measurements beside an aPTT.

Zusammenfassung

Clot-Waveform-Analyse leitet zusätzlich Daten aus aPTT-Messungen aus. Die aufgezeichnete Messkurve wird mathematisch aufbereitet und die Kinetik der Fibrinbildung einschließlich Thrombin-, Prothrombinaseund Tenaseaktivität semiquantitativ bestimmt. Patienten, Methode: Proben gesunder Blutspender als auch von Hämophilie-A-Patienten in verschiedenen klinischen Situationen wurden untersucht, um die Anwendbarkeit der Clot-Waveform-Analyse für die Überwachung der Hämophilie A zu prüfen. Ergebnisse: Thrombin-, Prothrombinaseund Tenaseaktivität zeigen eine hohe Korrelation zum Faktor-VIII-Spiegel. Ein linearer Zusammenhang wurde zwischen Tenaseaktivität und Faktor VIII-Spiegel über einen großen Konzentrationsbereich gefunden. Patienten mit schwerer, moderater oder milder Hämophilie können gut diskriminiert werden. Schlusssfolgerung: Clot-Waveform-Analyse ist eine einfache, schnelle und preiswerte Methode, um Störungen der Fibrinbildung zu untersuchen. Die Daten können aus einer aPTT-Bestimmung gewonnen werden.

Keywords

Activated partial thromboplastin time - fibrin formation kinetics - thrombin - prothrombinase - tenase - haemophilia

Schlüsselwörter

Aktivierte partielle Thromboplastinzeit - Fibrinbildungskinetik - Thrombin - Prothrombinase - Tenase - Hämophilie

Full Text

References

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